An inconvenient truth about xylem resistance to embolism in the model species for refilling Laurus nobilis L.

Laurent J. Lamarque (1, 2) , Déborah Corso (2) , Jose Manuel Torres Ruiz (2) , Eric Badel , Timothy J. Brodribb , Régis Burlett (2) , Guillaume Charrier (2, 1) , Brendan Choat , Hervé Cochard , Gregory A. Gambetta (1) , Steven Jansen , Andrew King (3) , Nicolas Lenoir , Nicolas Martin-StPaul (4) , Kathy Steppe , Jan van den Bulcke , Ya Zhang , Sylvain Delzon (2)

1 UMR EGFV - Ecophysiologie et Génomique Fonctionnelle de la Vigne
2 BioGeCo - Biodiversité, Gènes & Communautés
3 SSOLEIL - Synchrotron SOLEIL
4 URFM - Unité de Recherches Forestières Méditerranéennes

AbstractKey messageDirect, non-invasive X-ray microtomography and optical technique observations applied in stems and leaves of intact seedlings revealed that laurel is highly resistant to drought-induced xylem embolism. Contrary to what has been brought forward, daily cycles of embolism formation and refilling are unlikely to occur in this species and to explain how it copes with drought.ContextThere has been considerable controversy regarding xylem embolism resistance for long-vesselled angiosperm species and particularly for the model species for refilling (Laurus nobilis L.).AimsThe purpose of this study was to resolve the hydraulic properties of this species by documenting vulnerability curves of different organs in intact plants.MethodsHere, we applied a direct, non-invasive method to visualize xylem embolism in stems and leaves of intact laurel seedlings up to 2-m tall using X-ray microtomography (microCT) observations and the optical vulnerability technique. These approaches were coupled with complementary centrifugation measurements performed on 1-m long branches sampled from adult trees and compared with additional microCT analyses carried out on 80-cm cut branches.ResultsDirect observations of embolism spread during desiccation of intact laurels revealed that 50% loss of xylem conductivity (Ψ50) was reached at − 7.9 ± 0.5 and − 8.4 ± 0.3 MPa in stems and leaves, respectively, while the minimum xylem water potentials measured in the field were − 4.2 MPa during a moderate drought season. Those findings reveal that embolism formation is not routine in Laurus nobilis contrary to what has been previously reported. These Ψ50 values were close to those based on the flow-centrifuge technique (− 9.2 ± 0.2 MPa), but at odds with microCT observations of cut branches (− 4.0 ± 0.5 MPa).ConclusionIn summary, independent methods converge toward the same conclusion that laurel is highly resistant to xylem embolism regardless its development stage. Under typical growth conditions without extreme drought events, this species maintains positive hydraulic safety margin, while daily cycles of embolism formation and refilling are unlikely to occur in this species.

Mots clés: Desiccation; Xylem embolism; Drought resistance; Laurel; Refilling; Hydraulics

Domaines: Sciences du Vivant [q-bio]

Laurent J. Lamarque, Déborah Corso, Jose Manuel Torres Ruiz, Eric Badel, Timothy J. Brodribb, et al.. An inconvenient truth about xylem resistance to embolism in the model species for refilling Laurus nobilis L.. Annals of Forest Science, 2018, 75 (3), pp.88. ⟨10.1007/s13595-018-0768-9⟩. ⟨hal-02288736⟩